Cell Biology Methods - Essay Sample

Cell biology is the study of cells and how they function, the subcellular processes that keep them functioning, and how cells interact with other cells. Although molecular biology focuses mainly on life molecules (most nucleic acids and proteins), cell biology deals with the growth, reproduction, and efficiency of normal cell function. Cell biology is used in biomedical research to learn more about how cells function naturally and how diseases can arise from defects in this ordinary phase. Viewing these mechanisms will lead to strategies that will address the unstable function. The article discusses key cell biology methods, such as cell tissue and others.

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As it is the case in bacteria and other basic organisms, cells and tissues from more complex organisms may be cultivated in their natural habitat. The techniques are quite different, and the media of culture are more complex in representing the complex internal environment in which the cells are generated from the host. However, cells and tissues' culture is an effective instrument and provides researchers with almost infinite test material for use without the use of whole organisms. Furthermore, regulated conditions in the tissue and cell culture enable researchers to experiment with a reduced number of different variables that may impact the test outcomes. Cell culture may use lines of cultured cancer cells or cells taken directly from an organism (primary culture). The advantage of the above is the ongoing differentiation of cancer cells when primary cultures avoid dividing for years.

Microscopy – microscopy is the main device in cell biology. The latest advancements in image processing have made microscopic analysis possible to glean an unparalleled quantity of details.

RNA Interference – RNA interference is based on short RNA sequences that complement the mRNA that provides instructions for translating DNA proteins into the ribosome. The RNA interferes with the target sequence such that it is not interpreted. Thus diligent

Interfering RNA selection can be used to silence a single gene. Researchers will thus research the function of a protein in the cell by looking at what happens when the protein is missing.

Time-lapse microscopy– A variety of cellular processes (eg, mitosis) occurs over a period that is not feasible to observe directly. Imaging cells for some time (for example, every 20 minutes for 24 hours, photos are taken) enables one to merge them into a long-term "film" duration to shorter. Duration to shorter.

It can be summed up that most cell cycles revolve from G1 to G0, where their regular cellular functions are done, as most cells do not continuously need to divide. Changes in the cell cycle can lead to cells dividing continuously and can proceed to cancer. Understanding the cell cycle mechanisms will lead to curing cancer by either disrupting cancer cell cycles or preventing the conversion of cells into cancer. The article suggests that much more research has to be done on the cell cycle and mitosis area to enhance the clear envisioning of cell multiplication within a body.

Reference

Cruz-Orive, L. M., & Weibel, E. R. (1990). Recent stereological methods for cell biology: a brief survey. American Journal of Physiology-Lung Cellular and Molecular Physiology, 258(4), L148-L156.